Yeast chromosome replication and segregation
نویسندگان
چکیده
منابع مشابه
Dynamics of Escherichia coli chromosome segregation during multifork replication.
Slowly growing Escherichia coli cells have a simple cell cycle, with replication and progressive segregation of the chromosome completed before cell division. In rapidly growing cells, initiation of replication occurs before the previous replication rounds are complete. At cell division, the chromosomes contain multiple replication forks and must be segregated while this complex pattern of repl...
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In meiosis I of most organisms, homologous chromosomes pair, recombine, and then segregate to opposite poles of the cell. Crossing-over is normally necessary to ensure the proper segregation of the homologs. Recently developed techniques have made it possible to study meiosis with highly defined artificial chromosomes. These techniques were used to demonstrate the existence of a system capable ...
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We propose a new method for segregation of yeast artificial chromosomes (YACs) from endogenous yeast chromosomes with similar lengths. The method is based on recently developed PNA-assisted rare cleavage (PARC) of genomic DNA. We apply the PARC procedure to YAC-containing samples of yeast DNA in such a way that host chromosomes, which electrophoretically comigrate with the chosen YACs, are sele...
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Characterized bacteria, unlike eukaryotes and some archaea, initiate replication bidirectionally from a single replication origin contained within a circular or linear chromosome. We constructed Escherichia coli cells with two WT origins separated by 1 Mb in their 4.64-Mb chromosome. Productive bidirectional replication initiated synchronously at both spatially separate origins. Newly replicate...
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A surveillance mechanism, the S phase checkpoint, blocks progression into mitosis in response to DNA damage and replication stress. Segregation of damaged or incompletely replicated chromosomes results in genomic instability. In humans, the S phase checkpoint has been shown to constitute an anti-cancer barrier. Inhibition of mitotic cyclin dependent kinase (M-CDK) activity by Wee1 kinases is cr...
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ژورنال
عنوان ژورنال: Microbiological Reviews
سال: 1988
ISSN: 0146-0749
DOI: 10.1128/mr.52.4.568-601.1988